Anti-NOXO1 Antibody - Western Blot. Western blot of Affinity Purified anti-NOXO1 antibody shows detection of a band ~50 kD corresponding to human NOXO1 (arrowhead). Reactivity was observed in transfected human 293 cells and human HT-29 colon carcinoma cells (endogenous). Under these conditions endogenous NOXO1 detection was not observed in HeLa, HL-60, untransfected 293 or WT MEF cells. A 1:1000 dilution of the primary antibody was used for detection followed by secondary antibody reactivity. Specific band reactivity was competed away when the antibody was pre-incubated with the peptide immunogen (data not shown). Personal Communication, Zhenggang Liu, NIH, CCR, Bethesda, MD.