Confocal immunofluorescent of NRAS Antibody with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Western blot of anti-NRAS Antibody in CEM cell line lysates (35 ug/lane). NRAS(arrow) was detected using the purified antibody.
Western blot of NRAS (arrow) using rabbit polyclonal NRAS Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NRAS gene (Lane 2) (Origene Technologies).
NRAS Antibody flow cytometry of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.