Western blot analysis of extracts from Jurkat cells, untreated or treated with TPA #4174 and calcium ionophore A23187 for 4 hours, using Phospho-Nur77 (Ser351) (D22G5) Rabbit mAb. Antibody phospho-specificity was confirmed by pre-incubating the antibody with either a phosphorylated site-specific peptide to block the signal or a non-phosphorylated site-specific peptide that does not block the signal.
Western blot analysis of extracts from Jurkat cells, untreated or treated with TPA #4174 and calcium ionophore A23187 for the indicated times, using Phospho-Nur77 (Ser351) (D22G5) Rabbit mAb (upper) or Nur77 (D63C5) XP ® Rabbit mAb #3960 (lower).