Western blot analysis of extracts from COS-7 cells, untransfected (-) or transfected (+) with a human Nur77 construct, using Nur77 (D63C5) XP ® Rabbit mAb.
Western blot analysis of of extracts from Jurkat cells, untreated (-) or treated with TPA #4174 (40 nM) and A23187 (2 μM), using Nur77 (D63C5) XP ® Rabbit mAb.
Confocal immunofluorescent analysis of Jurkat cells, untreated (left) or treated with TPA #4174 and A23187 (right), using Nur77 (D63C5) XP ® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).
Flow cytometric analysis of Jurkat cells, untreated (blue) or treated with TPA #4174 and A23187 (green), using Nur77 (D63C5) XP ® Rabbit mAb.