Western blot analysis of NCCIT and NTERA2 cell extracts using Oct-4 Antibody.
Immunohistochemical analysis of paraffin-embedded human seminoma (left) or normal testis (right), showing nuclear localization using Oct-4 Antibody.
Confocal immunofluorescent analysis of NCCIT cells using Oct-4 Antibody (green) and β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 555 Conjugate) #2116 (red).
Flow cytometric analysis of untreated NCCIT cells using Oct-4 Antibody (blue) compared to a nonspecific negative control antibody (red).
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 NCCIT cells and either 20 μl of Oct-4 Antibody #2750 or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP ® Human Oct-4 Promoter Primers #4641, SimpleChIP ® Human Sox2 Promoter Primers #4649, and SimpleChIP ® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.