Oct-4A (C52G3) Rabbit mAb

• Supplier: Cell Signaling Technology
• Catalog: 2890
• Prices: $246.00
• Sizes: 100 µl (10 western blots)
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: C52G3
• Applications: WB IHC-P ICC/IF FC ChIP
• Species Reactivities: Human
• Antigen: Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human Oct-4A.
• Description: Rabbit Monoclonal
• Gene: POU5F1

Product images

Western blot analysis of extracts from NCCIT and NTERA2 cells using Oct-4A (C52G3) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded NTERA2 (positive, left) and HeLa (negative, right) cell pellets using Oct-4A (C52G3) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human seminoma, showing nuclear localization, using Oct-4A (C52G3) Rabbit mAb.

Confocal immunofluorescent analysis of NTERA2 cells using Oct-4A (C52G3) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).

Flow cytometric analysis of HeLa cells (blue) and NCCIT cells (green) using Oct-4A (C52G3) Rabbit mAb.

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 NCCIT cells and either 10 μl of Oct-4A (C52G3) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP ® Human Oct-4 Promoter Primers #4641, SimpleChIP ® Human Sox2 Promoter Primers #4649, and SimpleChIP ® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.