Formalin-fixed and paraffin-embedded human skin tissue reacted with SPARC Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot of SPARC (arrow) using rabbit polyclonal SPARC Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SPARC gene.
Western blot of SPARC Antibody in Y79 cell line lysates (35 ug/lane). SPARC (arrow) was detected using the purified antibody.
Flow cytometric of widr cells using SPARC Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.