Western blot analysis of extracts from human small intestine, colon, and prostate, and two human cancer cell lines (OV-90 and MCF7), using OLFM4 (D1E4M) XP ® Rabbit mAb (upper) and β-actin (D6A8) Rabbit mAb #8457 (lower). As expected, OLFM4 protein expression is not detected in MCF7 cells.
Immunohistochemical analysis of paraffin-embedded normal human colon using OLFM4 (D1E4M) XP ® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, stage I (left) and stage IV (right) using OLFM4 (D1E4M) XP ® Rabbit mAb. Note the stronger expression in the tumor of the stage I case compared to the stage IV case.
Immunohistochemical analysis of paraffin-embedded human ovarian serous cystadenoma using OLFM4 (D1E4M) XP ® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using OLFM4 (D1E4M) XP ® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).
Immunohistochemical analysis of paraffin-embedded human prostate adenocarcinoma using OLFM4 (D1E4M) XP ® Rabbit mAb.