Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Immunohistochemical of paraffin-embedded H.stomach section using PACSIN2 Antibody. Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Immunofluorescent of HeLa cells, using PACSIN2 Antibody. Antibody was diluted at 1:100 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor 554 (red) conjugated Phalloidin (red).
PACSIN2 Antibody (S357) western blot of Daudi cell line and mouse brain lysates (35 ug/lane). The PACSIN2 antibody detected the PACSIN2 protein (arrow).
PACSIN2 Antibody (S357) western blot of Daudi,HL-60 cell line and mouse brain,rat heart lysates (35 ug/lane). The PACSIN2 antibody detected the PACSIN2 protein (arrow).