p130 Cas (E1L9H) Rabbit mAb

• Supplier: Cell Signaling Technology
• Catalog: 13846
• Prices: $246.00
• Sizes: 100 µl (10 western blots)
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: E1L9H
• Applications: WB IP IHC-P ICC/IF FC
• Species Reactivities: Human, Mouse, Rat, Monkey
• Antigen: Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu116 in the SH3 domain of human p130 Cas protein.
• Description: Rabbit Monoclonal
• Gene: BCAR1

Product images

Western blot analysis of extracts from various cell lines using p130 Cas (E1L9H) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Immunoprecipitation of p130 Cas from Hep G2 cell extracts using Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (lane 2) or p130 Cas (E1L9H) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using p130 Cas (E1L9H) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using p130 Cas (E1L9H) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded T-47D (left) and Jurkat (right) cell pellets using p130 Cas (E1L9H) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using p130 Cas (E1L9H) Rabbit mAb in the presence of control peptide (left) and antigen-specific peptide (right).

Confocal immunofluorescent analysis of SNB19 cells either untreated (left) or treated with TPA #9905 (100nM, 1hr; right) using p130 Cas (E1L9H) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye).

Flow cytometric analysis of HT-1080 cells (green) and Jurkat cells (blue) using p130 Cas (E1L9H) Rabbit mAb. Anti-rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as secondary antibody.