Detection of Human PTIP by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-PTIP antibody used for IP at 6 ug/mg lysate. PTIP was also immunoprecipitated by a previous lot of this antibody. For blotting immunoprecipitated PTIP, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of Human PTIP by Western Blot. Samples: Whole cell lysate from 293T (15 and 50 ug), HeLa (50 ug), and Jurkat cells. Antibodies: Affinity purified rabbit anti-PTIP antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.