Phospho-p53 (Ser15) Antibody

• Supplier: Cell Signaling Technology
• Catalog: 9284
• Prices: $109.00, $287.00, $678.00
• Sizes: 20 µl (2 western blots), 100 µl (10 western blots), 300 µl (30 western blots)
• Host: Rabbit
• Clonality: Polyclonal
• Applications: WB IP ChIP
• Species Reactivities: Human, Mouse, Rat, Monkey, Mink, Bovine, Pig
• Antigen: Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser15 of human p53
• Description: Rabbit Polyclonal
• Gene: TP53

Product images

Western blot analysis of extracts from MvILu cells treated with UV or hydroxyurea (20 mM) for the indicated times, using Phospho-p53 (Ser15) Antibody.

Western blot analysis of a p53 fusion protein, untreated or phosphorylated by DNA-PK, using Phospho-p53 (Ser15) Antibody (upper) and p53 Antibody #9282 (lower).

Western blot analysis of extracts from PC12 cells treated with UV for the indicated times, using Phospho-p53 (Ser15) Antibody.

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 HCT116 cells treated with UV (100 J/m 2 followed by a 3 hour recovery) and either 5 μl of Phospho-p53 (Ser15) Antibody or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP ® Human CDKN1A Promoter Primers #6449, human MDM2 intron 2 primers, and SimpleChIP ® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.