Western blot analysis of extracts from HT29 cells treated with nocodazole (50 ng/ml, 24h), calyculin A #9902 (100 nM, 10 min), or lambda Phosphatase NEB#P0753 (10,000 units/ml, 1 h), using Phospho-p53 (Ser33) Antibody (upper) or p53 (1C12) Mouse mAb #2524 (lower).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Phospho-p53 (Ser33) Antibody.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, showing nuclear localization, using Phospho-p53 (Ser33) Antibody.
Immunohistochemical analysis of paraffin-embedded human lung dysplasia of alveolar cells, using Phospho-p53 (Ser33) Antibody in the presence of control peptide (left) or antigen specific peptide (right),