Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot of PDK2 Antibody in mouse intestine tissue lysate (35 ug/lane). PDK2 (arrow) was detected using the purified antibody.
PDK2 Antibody (C392) western blot of HeLa cell line lysates (35 ug/lane). The PDK2 antibody detected the PDK2 protein (arrow).
Western blot of PDK2 (arrow) using PDK2 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PDK2 gene (Lane 2) (Origene Technologies).