Anti-PDK4 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3459 concentration 10 ug/ml.
Anti-PDK4 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3459 concentration 10 ug/ml.
Fluorescent confocal image of HeLa cells stained with PDK4 antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated PDK4 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the PDK4 mainly to the cytoplasm.
PDK4 Antibody (C396) western blot of K562 cell line lysates (35 ug/lane). The PDK4 antibody detected the PDK4 protein (arrow).
The PDK4 Antibody is used in Western blot to detect PDK4 in mouse skeletal muscle tissue lysate.