Western blot analysis of extracts from ME-180 (+), HaCaT (+), and MCF7 (-) cells using p63-α (D2K8X) XP ® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Immunoprecipitation of p63-α from Me180 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or p63-α (D2K8X) XP® Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot was performed using p63-alpha (D2K8X) XP® Rabbit mAb.
Confocal immunofluorescent analysis of ME-180 (left) and MCF7 (right) cells using p63-α (D2K8X) XP ® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye).
Flow cytometric analysis of ME-180 (green) and MCF7 (blue) cells using p63-α (D2K8X) XP ® Rabbit mAb. Anti-rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 488 Conjugate) #4412 was used as a secondary antibody.
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 HaCaT cells and either 5 μl of p63-α (D2K8X) XP® Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP ® Human CDKN1A Promoter Primers #6449, human MDM2 intron 2 primers, and SimpleChIP ® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.