Formalin-fixed and paraffin-embedded human testis tissue reacted with PFTK1 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot of lysate from human brain tissue lysate, using PFTK1 Antibody (N-term ). Antibody was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
Western blot of PFTK1 antibody in mouse cerebellum tissue lysates (35 ug/lane). PFTK1 (arrow) was detected using the purified antibody.
Western blot of lysates from HeLa cell line , human brain and mouse cerebellum tissue lysate(from left to right), using PFTK1 Antibody (N-term ). Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.