Western blot analysis of MCF7 cells, untreated (-) or doxorubicin-treated (0.5 μM, 24 hr; +), using p73 (D3G10) Rabbit mAb (upper) and α-Actinin (D6F6) XP ® Rabbit mAb #6487 (lower).
Western blot analysis of HCT 116 cells, untreated (-) or treated with Etoposide #2200 (25 μM, 24 hr; +), using p73 (D3G10) Rabbit mAb (upper) and α-Actinin (D6F6) XP ® Rabbit mAb #6487 (lower).
Immunoprecipitation of p73 from doxorubicin-treated (0.5 μM, 24 hr) MCF7 cell extracts using Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (lane 2) or p73 (D3G10) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot was performed using p73 (D3G10) Rabbit mAb.
Confocal immunofluorescent analysis of HCT 116 cells, untreated (left) or treated with Etoposide #2200 (25 μM, 24 hr; right), using p73 (D3G10) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red).