Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Negative control of HeLa cells without Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Immunofluorescence of PI4K2A (PI4K II) Antibody in HeLa cells. 0.025 mg/ml primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L).
Western blot of anti-PI4K2A Antibody (N-term D2) in mouse brain tissue lysates (35 ug/lane). PI4KII(arrow) was detected using the purified antibody.
Western blot of anti-PI4K II antibody in HL60 cell lysate. PI4K II (arrow) was detected using purified antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.