Western blot detection of eIF4E phosphorylation in NIH/3T3 cells that were serum starved and then treated with serum. Blots were probed with eIF4E (P-2): sc-9976 (A) and p-eIF4E (Ser 209)-R: sc-12885-R (B,C). Antibody was preincubated with cognate non-phosphorylated (B) or phosphorylated (C) peptide.
Western blot analysis of phosphorylated elF4E expression in untreated (A,D), insulin-treated (B,E) and serum starved then, serum treated (C,F) NIH/3T3 whole cell lysates. Blots were probed with p-eIF4E (Ser 209)-R: sc-12885-R (A,B,C) and eIF4E (FL-217): sc-13963 preincubated with its cognate phosphorylated peptide (D,E,F).
p-eIF4E Antibody (Ser 209): sc-12885-R. Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing cytoplasmic staining of exocrine glandular cells and Islets of Langerhans.