Formalin-fixed and paraffin-embedded human lung carcinoma reacted with PIN1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot of PIN1 (arrow) using rabbit polyclonal PIN1 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PIN1 gene.
Western blot of PIN1 Antibody in HL-60 cell line lysates (35 ug/lane). PIN1 (arrow) was detected using the purified antibody.
PIN1 Antibody FC of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.