PIN1

Gene Symbol PIN1
Entrez Gene 5300
Alt Symbol DOD, UBL5
Species Human
Gene Type protein-coding
Description peptidylprolyl cis/trans isomerase, NIMA-interacting 1
Other Description PPIase Pin1|peptidyl-prolyl cis-trans isomerase NIMA-interacting 1|protein (peptidyl-prolyl cis/trans isomerase) NIMA-interacting 1|rotamase Pin1
Swissprots A8K4V9 Q13526 Q53X75
Accessions AAM81970 EAW84057 EAW84058 EAW84059 Q13526 AF086512 AK092970 AK097558 BAG53486 AK291074 BAF83763 BC002899 AAH02899 BC031971 AAH31971 BG912418 BI827520 BI827672 BT019331 AAV38138 CA439440 CD675162 CR407654 CAG28582 DQ892978 ABM83904 DQ896226 ABM87225 U49070 AAC50492 XM_011528068 XP_011526370 NM_006221 NP_006212 NR_038422 NR_038830
Function Peptidyl-prolyl cis/trans isomerase (PPIase) that binds to and isomerizes specific phosphorylated Ser/Thr-Pro (pSer/Thr- Pro) motifs in a subset of proteins, resulting in conformational changes in the proteins (PubMed:21497122, PubMed:22033920). Displays a preference for an acidic residue N-terminal to the isomerized proline bond. Regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Down-regulates kinase activity of BTK (PubMed:16644721). Can transactivate multiple oncogenes and induce centrosome amplification, chromosome instability and cell transformation. Required for the efficient dephosphorylation and recycling of RAF1 after mitogen activation (PubMed:15664191). Binds and targets PML and BCL6 for degradation in a phosphorylation-dependent manner (PubMed:17828269). Acts as a regulator of JNK cascade by binding to phosphorylated FBXW7, disrupting FBXW7 dimerization and promoting FBXW7 autoubiquitination and degradation: degradation of
Subcellular Location Nucleus {ECO:0000269|PubMed:16476580}. Nucleus speckle {ECO:0000269|PubMed:21497122}. Cytoplasm {ECO:0000269|PubMed:21497122}. Note=Colocalizes with NEK6 in the nucleus (PubMed:16476580). Mainly localized in the nucleus but phosphorylation at Ser-71 by DAPK1 results in inhibition of its nuclear localization (PubMed:21497122). {ECO:0000269|PubMed:16476580}.
Tissue Specificity The phosphorylated form at Ser-71 is expressed in normal breast tissue cells but not in breast cancer cells. {ECO:0000269|PubMed:17828269, ECO:0000269|PubMed:21497122}.
Top Pathways RIG-I-like receptor signaling pathway

Pin1 Antibody - 3722 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HeLa, NIH/3T3, PC12 and COS cells, using Pin1 Antibody.

Phospho-Pin1 (Ser16) Antibody - 3721 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal

Pin1 (E-5) - sc-365028 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Pin1 (E-5): sc-365028. Western blot analysis of Pin1 expression in non-transfected: sc-117752 (A) and mouse Pin1 transfected: sc-122584 (B) 293T whole cell lysates.

Pin1 (F-7) - sc-271441 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Pin1 (F-7): sc-271441. Western blot analysis of Pin1 expression in non-transfected: sc-117752 (A) and mouse Pin1 transfected: sc-122584 (B) 293T whole cell lysates.

Pin1 (C-20) - sc-7409 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Pin1 (C-20): sc-7409. Western blot analysis of Pin1 expression in K-562 (A), Jurkat (B) and PC-3 (C) nuclear extracts.

Pin1 (A-20) - sc-7412 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Pin1 (A-20): sc-7412. Western blot analysis of Pin1 expression in non-transfected: sc-117752 (A) and mouse Pin1 transfected: sc-122584 (B) 293T whole cell lysates.

Pin1 (H-123) - sc-15340 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Pin1 (H-123): sc-15340. Western blot analysis of Pin1 expression in HeLa (A), K-562 (B) and Jurkat (C) nuclear extracts.

Pin1 (N-19) - sc-7413 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Pin1 (G-8) - sc-46660 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Pin1 (G-8): sc-46660. Immunoperoxidase staining of formalin fixed, paraffin-embedded human fallopian tube tissue showing nuclear staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.

Pin1 (8C10) - sc-81533 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
Pin1 (8C10): sc-81533. Western blot analysis of Pin1 expression in serum starved A-431 (A), A549 (B), SKOV3 (C), OVCAR-5 (D), HaCaT (E), PC-3 (F), HeLa (G) and Hep G2 (H) whole cell lysates.

Anti-Pin1 (phospho S16) antibody - ab138674 from Abcam

Host Rabbit
Clonality Polyclonal
Anti-Pin1 (phospho S16) antibody (ab138674) at 1/500 dilution + COS7 cell lysate (treated with 0.01 U/ml insulin for 15 minutes) at 30 µg

Anti-Pin1 (phospho S16) antibody [EP1480Y] - ab62378 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1480Y
All lanes : Anti-Pin1 (phospho S16) antibody [EP1480Y] (ab62378) at 1/500 dilutionLane 1 : HeLa cell lysate (untreated)Lane 2 : HeLa cell lysate (treated with FBS + Calyculin A)Lysates/proteins at 10 µg per lane.SecondaryGoat anti-Rabbit HRP conjugated at 1/2000 dilution

Anti-Pin1 antibody - ab12107 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Lane 1 : MarkerLanes 2 - 3 : Anti-Pin1 antibody (ab12107) at 1 µg/mlLane 1 : As aboveLane 2 : pin1 Recombinant 0.1ugLane 3 : pin1 Recombinant 0.5ugSecondaryLanes 2 - 3 : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7090) at 1/10000 dilutionPerformed under reducing conditions.Observed band size : ~34 kDa (why is the actual band size different from the predicted?)

Anti-Pin1 antibody - ab63623 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunofluorescence analysis of NIH/3T3 cells, using ab63623 at a dilution of 1/500-1/1000. Samples were untreated (left hand panel) or treated (right hand panel) with the immunizing peptide.

Anti-Pin1 antibody - ab37891 from Abcam

Prices $376.00
Sizes 50 µg
Host Chicken
Clonality Polyclonal
Anti-Pin1 antibody (ab37891) at 1/2000 dilution + E coli-derived fusion proteinSecondarygoat anti-IgY-HRP at 1/1000 dilution

Anti-Pin1 antibody [3G8] - ab38933 from Abcam

Prices $376.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 3G8
All lanes : Anti-Pin1 antibody [3G8] (ab38933) at 1/500 dilutionLane 1 : 293T cell lysateLane 2 : HeLa cell lysateLane 3 : Jurkat cell lysateLysates/proteins at 50 µg per lane.Secondarygoat anti-mouse secondary antibody conjugated to HRPdeveloped using the ECL technique

Anti-Pin1 antibody (Biotin) - ab118604 from Abcam

Prices $370.00
Sizes 150 µg
Host Rabbit
Clonality Polyclonal

Anti-Pin1 antibody [EP1479Y] - ab76309 from Abcam

Prices $391.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1479Y
Anti-Pin1 antibody [EP1479Y] (ab76309) at 1/500 dilution + 293 cell lysate at 10 µgSecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-Pin1 antibody - N-terminal - ab189321 from Abcam

Prices $376.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunofluorescent analysis of NIH 3T3 cells labeling Pin1 with ab189321 at 1/100 dilution, in the presence (right panel) or absence (left panel) of immunizing peptide.

Anti-PIN1 Antibody (C-Terminus) - LS-C9615 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-PIN1 Antibody - LS-C9614 from LifeSpan Bioscience

Prices $445.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-PIN1 Antibody - LS-C9616 from LifeSpan Bioscience

Prices $475.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal

Anti-PIN1 Antibody - LS-C9617 from LifeSpan Bioscience

Prices $355.00
Sizes 50 µl
Host Mouse
Clonality Monoclonal

Anti-PIN1 Antibody (aa50-100) - LS-C91914 from LifeSpan Bioscience

Prices $295.00
Sizes 40 µg
Host Rabbit
Clonality Polyclonal

Anti-PIN1 Antibody (phospho-Ser16, clone EP1480Y) - LS-C50105 from LifeSpan Bioscience

Prices $450.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1480Y
Western blot analysis on HeLa cell lysates using anti-Phospho-Pin1 (pS16) antibody, 1:500 dilution. Cells were either (A) untreated or (B) treated with FBS + Calyculin A.
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