Anti-PKM / Pyruvate Kinase, Muscle Antibody (aa476-505)
| Name | Anti-PKM / Pyruvate Kinase, Muscle Antibody (aa476-505) |
|---|---|
| Supplier | LifeSpan Bioscience |
| Catalog | LS-C98973 |
| Prices | $295.00 |
| Sizes | 400 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Applications | IHC-P WB IP ELISA |
| Species Reactivities | Rabbit, Human |
| Purity/Format | Ammonium sulfate precipitation |
| Blocking Peptide | PKLR Antibody Blocking Peptide |
| Description | Rabbit Polyclonal |
| Gene | PKM |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Pyruvate Kinase (PKM2) Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human hepatocarcinoma followed by peroxidase-conjugated secondary antibody and DAB staining. This data demonstrates the use of the Pyruvate Kinase (PKM2) Antibody for immunohistochemistry.
Fluorescent confocal image of MCF7 cells stained with Pyruvate Kinase (PKM2) antibody. MCF7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated Pyruvate Kinase (PKM2) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the Pyruvate Kinase (PKM2) mainly to the cytoplasm, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000067225).
Western blot of anti-h PKM2-N491 antibody in Ramos cell line lysates (35 ug/lane). hPKM2-N491(arrow) was detected using the purified antibody.