Anti-POLK antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5464 concentration 5 ug/ml.
Immunofluorescence of monoclonal antibody to POLK on HeLa cell. [antibody concentration 10 ug/ml]
Western blot of POLK expression in HeLa nuclear extracts by POLK monoclonal antibody, clone 6F2.
Western blot of POLK expression in transfected 293T cell line by POLK monoclonal antibody, clone 6F2.
Detection limit for recombinant GST tagged POLK is approximately 0.3 ng/ml as a capture antibody.
Western blot of POLK over-expressed 293 cell line, cotransfected with POLK Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with POLK monoclonal antibody, clone 6F2. GAPDH (36.1 kD) used as control.