Anti-POU5F1 / OCT4 Antibody (aa110-141)
| Name | Anti-POU5F1 / OCT4 Antibody (aa110-141) |
|---|---|
| Supplier | LifeSpan Bioscience |
| Catalog | LS-C97712 |
| Prices | $295.00 |
| Sizes | 400 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Applications | IHC-P ICC/IF WB FC |
| Species Reactivities | Human |
| Purity/Format | Ammonium sulfate precipitation |
| Blocking Peptide | POU3F3 / BRN1 Antibody Blocking Peptide |
| Description | Rabbit Polyclonal |
| Gene | POU5F1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Fluorescent confocal image of SY5Y cells stained OCT4 (E125) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated OCT4 (E125) primary antibody (1:500, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 mu M, 25 min). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 3 min). OCT4 immunoreactivity is localized mainly to the nuclei and also to the cytoplasm.
Western blot of OCT3/4 Antibody (E125) in HL60 cell line lysates (35 ug/lane). OCT3/4 (arrow) was detected using the purified antibody.