PCNA (D3H8P) XP ® Rabbit mAb

• Supplier: Cell Signaling Technology
• Catalog: 13110
• Prices: $109.00, $282.00
• Sizes: 20 µl (2 western blots), 100 µl (10 western blots)
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: D3H8P
• Applications: WB IP IHC-P ICC/IF FC
• Species Reactivities: Human, Mouse, Rat, Monkey
• Antigen: Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human PCNA protein.
• Description: Rabbit Monoclonal
• Gene: PCNA

Product images

Western blot analysis of extracts from various cell lines using PCNA (D3H8P) XP ® Rabbit mAb.

Immunoprecipitation of PCNA from HeLa cell extracts using Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (lane 2) or PCNA (D3H8P) XP ® Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot was performed using PCNA (D3H8P) XP ® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using PCNA (D3H8P) XP® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using PCNA (D3H8P) XP ® Rabbit mAb.

Confocal immunofluorescent analysis of rat small intestine using PCNA (D3H8P) XP ® Rabbit mAb (green) showing staining of proliferative cells in intestinal crypts and β-Catenin (L54E2) Mouse mAb (IF Preferred) #2677 (red). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye).

Confocal immunofluorescent analysis of HeLa cells using PCNA (D3H8P) XP ® Rabbit mAb (green) and β-Actin (8H10D10) Mouse mAb #3700 (red).

Flow cytometric analysis of Jurkat cells using PCNA (D3H8P) XP ® Rabbit mAb and Propidium Iodide (PI)/RNase Staining Solution #4087 to measure DNA content. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor ® 488 Conjugate) #4412 was used as a secondary antibody.