PRDM14 in BG01 V Human Cell Line. PRDM14 was detected in fixed BG01 V human embryonic stem cells grown on irradiated MEF cells using Human PRDM14 Monoclonal Antibody at 10 ug/ml for 3 hours at room temperature. Cells were stained using NorthernLights 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei.
Detection of Human PRDM14 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF Membrane was probed with 2 ug/ml of Human PRDM14 Monoclonal Antibody followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody. A specific band was detected for PRDM14 at approximately 80 kD (as indicated). This experiment was conducted under reducing conditions.