PRDM14

Gene Symbol	PRDM14
Entrez Gene	63978
Alt Symbol	PFM11
Species	Human
Gene Type	protein-coding
Description	PR domain containing 14
Other Description	PR domain zinc finger protein 14\|PR domain-containing 14\|PR domain-containing protein 14
Swissprots	Q86UX9 Q9GZV8
Accessions	EAW86962 Q9GZV8 AF319458 AAG39635 AK022595 BAB14120 BC052311 AAH52311 BF770200 CX166395 CX788380 EU446841 ABZ92370 HQ257953 ADR82707 KJ463837 AHX99430 XM_011517572 XP_011515874 NM_024504 NP_078780
Function	Transcription factor that has both positive and negative roles on transcription. Required for the maintenance of emryonic stem cell identity and the reacquisition of pluripotency in somatic cells. May play an essential role in germ cell development at 2 levels: the reacquisition of potential pluripotency, including SOX2 up-regulation, and successful epigenetic reprogramming, characterized by EHMT1 repression (By similarity). Directly up-regulates the expression of pluripotency gene POU5F1 through its proximal enhancer. Binds to the DNA consensus sequence 5'-GGTC[TC]CTAA-3'. {ECO:0000250, ECO:0000269\|PubMed:17942894, ECO:0000269\|PubMed:20953172}.
Subcellular Location	Nucleus {ECO:0000269\|PubMed:17942894}.
Tissue Specificity	Expressed in embryonic stem cells. Tends to be overexpressed in breast cancer (at protein level). {ECO:0000269\|PubMed:17942894, ECO:0000269\|PubMed:20953172}.

PRDM14 (H-119) - sc-367835 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Rabbit
Clonality	Polyclonal

PRDM14 (N-14) - sc-87368 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	100 µg/ml
Host	Rabbit
Clonality	Polyclonal

PRDM14 (K-23) - sc-133923 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	100 µg/ml
Host	Rabbit
Clonality	Polyclonal

PRDM14 (K-23): sc-133923. Western blot analysis of PRDM14 expression in Jurkat whole cell lysate.

Anti-PRDM14 antibody - ab187881 from Abcam

Prices	$370.00
Sizes	50 µl
Host	Rabbit
Clonality	Polyclonal

Immunocytochemistry/Immunofluorescence analysis of A549 cells using ab187881. Blue DAPI for nuclear staining.

Anti-PRDM14 antibody - ab192411 from Abcam

Prices	$370.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-PRDM14 antibody - ab91587 from Abcam

Prices	$370.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-PRDM14 antibody (ab91587) at 1 µg/mlLane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Lane 2 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate Lane 3 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate Lane 4 : HuES7 Whole Cell Lysate Lane 5 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate Lane 6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 7 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-PRDM14 Antibody (aa2-201) - LS-C124416 from LifeSpan Bioscience

Prices	$605.00
Sizes	100 µg
Host	Sheep
Clonality	Polyclonal

Anti-PRDM14 Antibody (aa414-427) - LS-C139755 from LifeSpan Bioscience

Prices	$335.00
Sizes	100 µg
Host	Goat
Clonality	Polyclonal

Anti-PRDM14 Antibody - LS-C144112 from LifeSpan Bioscience

Prices	$465.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal

Anti-PRDM14 Antibody (N-Terminus) - LS-C190135 from LifeSpan Bioscience

Prices	$495.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-PRDM14 Antibody (aa1-30, AP) - LS-C239403 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-PRDM14 Antibody (aa1-30, APC) - LS-C239404 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-PRDM14 Antibody (aa1-30, PE) - LS-C239408 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-PRDM14 Antibody (aa1-30, Biotin) - LS-C239405 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-PRDM14 Antibody (aa1-30, FITC) - LS-C239406 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-PRDM14 Antibody (aa1-30) - LS-C324275 from LifeSpan Bioscience

Prices	$345.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-PRDM14 Antibody (aa1-30, Azide-free, HRP) - LS-C248543 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-PRDM14 Antibody - LS-C341664 from LifeSpan Bioscience

Prices	$385.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-PRDM14 Antibody (aa208-257) - LS-C31290 from LifeSpan Bioscience

Prices	$325.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

PRDM14 antibody LS-C31290 was used in IHC to stain formalin-fixed, paraffin-embedded human lung.

Anti-PRDM14 Antibody - LS-C150037 from LifeSpan Bioscience

Prices	$395.00
Sizes	100 µg
Host	Sheep
Clonality	Polyclonal

Detection of PRDM14. Cell lysates were resolved by SDS-PAGE, transferred to an Immobilon-P membrane and immunoblotted with 0.1 ug/ml sheep anti-hPRDM14.

Anti-PRDM14 Antibody (aa533-544) - LS-C186585 from LifeSpan Bioscience

Prices	$395.00
Sizes	100 µg
Host	Goat
Clonality	Polyclonal

PRDM14 antibody (2 ug/ml) staining of A549 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-PRDM14 Antibody - LS-C335685 from LifeSpan Bioscience

Prices	$255.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Western blot analysis of extracts of various cell lines, using PRDM14 antibody.

Anti-PRDM14 Antibody (clone 647002) - LS-C150036 from LifeSpan Bioscience

Prices	$315.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	647002

PRDM14 in BG01 V Human Cell Line. PRDM14 was detected in fixed BG01 V human embryonic stem cells grown on irradiated MEF cells using Human PRDM14 Monoclonal Antibody at 10 ug/ml for 3 hours at room temperature. Cells were stained using NorthernLights 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei.

Anti-PRDM14 Antibody (aa1-30) - LS-C98753 from LifeSpan Bioscience

Prices	$295.00
Sizes	400 µl
Host	Rabbit
Clonality	Polyclonal

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Anti-PRDM14 Antibody - LS-C334124 from LifeSpan Bioscience

Prices	$255.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Western blot analysis of various groups from PA-1 cell line, using PRDM14 antibody. ShRNA-1, shRNA-2, shRNA-3, shRNA-4, shRNA-5, shRNA-6 show knock-down samples which are transfected by different shRNAs targeting PRDM14. Control represents the untreated one.

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