Immunofluorescence of monoclonal antibody to PRKCI on HeLa cell . [antibody concentration 10 ug/ml]
Western Blot analysis of PRKCI expression in transfected 293T cell line by PRKCI monoclonal antibody (M01), clone 1G11.Lane 1: PRKCI transfected lysate(68.3 KDa).Lane 2: Non-transfected lysate.
Detection limit for recombinant GST tagged PRKCI is approximately 3 ng/ml as a capture antibody.
Western blot analysis of PRKCI over-expressed 293 cell line, cotransfected with PRKCI Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with PRKCI monoclonal antibody (M01) clone 1G11 . GAPDH ( 36.1 kDa ) used as specificity and loading control.