Prolactin R in Rat Thymus. Prolactin R was detected in perfusion fixed frozen sections of rat thymus using Goat Anti-Rat Prolactin R Antigen Affinity-purified Polyclonal Antibody at 15 ug/ml overnight at 4°C. Tissue was stained using Anti-Goat HRP-DAB (brown) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of lymphocytes.
Cell Proliferation Induced by Prolactin and Neutralization by Rat Prolactin R Antibody. Recombinant Human Prolactin stimulates proliferation in the Nb2-11 rat lymphoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Prolactin (0.5 ng/ml) is neutralized (green line) by increasing concentrations of Goat Anti-Rat Prolactin R Antigen Affinity-purified Polyclonal Antibody. 25 ug/ml of this antibody will neutralize Recombinant Human Prolactin-induced activity by>60%.