Detection of Human PSME1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-PSME1 antibody used for IP at 6 ug/mg lysate. PSME1 was also immunoprecipitated by rabbit anti-PSME1 antibody BL13937. For blotting immunoprecipitated PSME1, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of Human PSME1 by Western Blot. Samples: Whole cell lysate from 293T (15 and 50 ug), HeLa (H; 50 ug), and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PSME1 antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.