Detection of Human PSME3 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-PSME3 antibody used for IP at 6 ug/mg lysate. For blotting immunoprecipitated , was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of Human and Mouse PSME3 by Western Blot. Samples: Whole cell lysate from 293T (15 and 50 ug), HeLa (50 ug), Jurkat (50 ug), and mouse NIH3T3 (50 ug) cells. Antibodies: Affinity purified rabbit anti-PSME3 antibody used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.