PSME3

Gene Symbol PSME3
Entrez Gene 10197
Alt Symbol HEL-S-283, Ki, PA28-gamma, PA28G, PA28gamma, REG-GAMMA
Species Human
Gene Type protein-coding
Description proteasome (prosome, macropain) activator subunit 3 (PA28 gamma; Ki)
Other Description 11S regulator complex gamma subunit|11S regulator complex subunit gamma|Ki antigen|Ki nuclear autoantigen|PA28 gamma variant 5|REG gamma-3|activator of multicatalytic protease subunit 3|epididymis secretory protein Li 283|proteasome activator 28 subunit gamma|proteasome activator 28-gamma|proteasome activator complex subunit 3
Swissprots O35563 A8K9A3 P61289 Q9BQD9 Q12920 P97373 Q13172
Accessions AAA93227 CAF86206 EAW60891 EAW60892 EAW60893 EAW60894 P61289 AI304773 AK057221 BAG51887 AK074999 BAG52046 AK292618 BAF85307 AK303211 BAH13919 AK307110 AK314347 BC001423 AAH01423 BC002684 AAH02684 BC008020 AAH08020 BG708969 BI464061 BQ005550 BT019386 AAV38193 BX640801 CAE45884 CR541745 CAG46545 DQ891930 ABM82856 DQ895117 ABM86043 EU794653 ACJ13707 FJ200488 ACO54885 JX156303 AFP49816 U11292 AAB60335 XM_011524177 XP_011522479 XM_011524178 XP_011522480 NM_001267045 NP_001253974 NM_005789 NP_005780 NM_176863 NP_789839 NR_049772
Function Subunit of the 11S REG-gamma (also called PA28-gamma) proteasome regulator, a doughnut-shaped homoheptamer which associates with the proteasome. 11S REG-gamma activates the trypsin-like catalytic subunit of the proteasome but inhibits the chymotrypsin-like and postglutamyl-preferring (PGPH) subunits. Facilitates the MDM2-p53/TP53 interaction which promotes ubiquitination- and MDM2-dependent proteasomal degradation of p53/TP53, limiting its accumulation and resulting in inhibited apoptosis after DNA damage. May also be involved in cell cycle regulation. {ECO:0000269|PubMed:10835274, ECO:0000269|PubMed:11185562, ECO:0000269|PubMed:11432824, ECO:0000269|PubMed:15111123, ECO:0000269|PubMed:18309296, ECO:0000269|PubMed:9325261}.
Subcellular Location Nucleus {ECO:0000269|PubMed:10657252, ECO:0000269|PubMed:12629132}. Cytoplasm {ECO:0000250}. Note=Localizes to the cytoplasm during mitosis following nuclear envelope breakdown at this distinct stage of the cell cycle which allows its interaction with MAP3K3 kinase. {ECO:0000250}.
Top Pathways Proteasome, Hepatitis C, Antigen processing and presentation

PA28γ Antibody - 2412 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from MCF-7, Neuro2A, PC12 and COS cells using PA28γ Antibody.

PA28γ (H-82) - sc-367087 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

PA28γ (N-17) - sc-21273 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
PA28γ (N-17): sc-21273. Western blot analysis of PA28g expression in HeLa (A) and TT (B) whole cell lysates.

PA28γ (L-16) - sc-21274 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

PA28γ (47) - sc-136025 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
PA28g (47): sc-136025. Western blot analysis of PA28g expression in rat cerebrum tissue extract.

PA28γ (Z-21) - sc-133876 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Rabbit
Clonality Polyclonal
PA28γ (Z-21): sc-133876. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human kidney tissue showing nuclear and cytoplasmic localization.

Anti-PSME3 antibody - ab97576 from Abcam

Host Rabbit
Clonality Polyclonal
All lanes : Anti-PSME3 antibody (ab97576) at 1/1000 dilutionLane 1 : A431 whole cell lysateLane 2 : H1299 whole cell lysateLysates/proteins at 30 µg per lane.

Anti-PSME3 antibody [4B2-C2-G4] - ab106213 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 4B2-C2-G4
All lanes : Anti-PSME3 antibody [4B2-C2-G4] (ab106213) at 1/1000 dilutionLane 1 : HepG2 whole cell lysateLane 2 : Jurkat whole cell lysateLane 3 : K562 whole cell lysateLysates/proteins at 40 µg per lane.

Anti-PSME3 antibody - C-terminal - ab91542 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-PSME3 antibody - C-terminal (ab91542) at 1/100 dilution + Ramos cell line lysates at 35 µg

Anti-PSME3 Antibody (aa48-97) IHC-plus™ - LS-B6840 from LifeSpan Bioscience

Prices $450.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-PSME3 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6840 concentration 5 ug/ml.

Anti-PSME3 Antibody (aa1-254) - LS-C185832 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
IHC of paraffin-embedded CA922 xenograft using PSME3 antibody at 1:100 dilution.

Anti-PSME3 Antibody (Internal) - LS-C38023 from LifeSpan Bioscience

Prices $465.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-PSME3 Antibody (N-Terminus) - LS-C38024 from LifeSpan Bioscience

Prices $345.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-PSME3 Antibody (HRP) - LS-C212067 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-PSME3 Antibody (C-Terminus) - LS-C55333 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Antibody (0.1 ug/ml) staining of K562 cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-PSME3 Antibody - LS-C212069 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-PSME3 Antibody (FITC) - LS-C212071 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-PSME3 Antibody (Biotin) - LS-C212072 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-PSME3 Antibody (C-Terminus) - LS-C80841 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
HepG2 cell lysate. Antibody concentration: 1.25 ug/ml. Gel concentration: 12%.

Anti-PSME3 Antibody - LS-C179890 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-PSME3 Antibody - LS-C192249 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (IHC) analysis of PSME3 pAb in paraffin-embedded Colon cancer tissue.

Anti-PSME3 Antibody - LS-C178263 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Western blot detection of PSME3 in HepG2 ,Jurkat&K562 whole cell lysate(1:1000 diluted).

Anti-PSME3 Antibody (aa204-254) - LS-C290529 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human PSME3 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-PSME3 antibody used for IP at 6 ug/mg lysate. For blotting immunoprecipitated , was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Anti-PSME3 Antibody - LS-C330887 from LifeSpan Bioscience

Prices $255.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry of paraffin-embedded human lung cancer using PSME3 antibody at dilution of 1:200 (x400 lens).

PA28 Activator gamma Subunit/PSME3 Antibody - NBP1-33498 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: PA28 Activator gamma Subunit/PSME3 Antibody [NBP1-33498] - Sample (30 ug of whole cell lysate) A: A549 B: H1299 C: HCT116 D: MCF-7 12% SDS PAGE, antibody diluted at 1:2000.
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