Detection of Human PYGL by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using RIPA buffer from 293T cells. Antibodies: Affinity purified rabbit anti-PYGL antibody used for IP at 6 ug per reaction. PYGL was also immunoprecipitated by rabbit anti-PYGL antibody BL15846. For blotting immunoprecipitated PYGL, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of Human and Mouse PYGL by Western Blot. Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-PYGL antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.