Anti-SPG7 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SPG7.
Immunofluorescent staining of HeLa cells using anti-SPG7 mouse monoclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPG7 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SPG7.
Western blot of extracts (35ug) from 9 different cell lines by using anti-SPG7 monoclonal antibody.
Western blot of extracts (10ug) from 10 Human tissue by using anti-SPG7 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).