PIAS3 (D5F9) XP ® Rabbit mAb

• Supplier: Cell Signaling Technology
• Catalog: 9042
• Prices: $109.00, $277.00
• Sizes: 20 µl (2 western blots), 100 µl (10 western blots)
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: D5F9
• Applications: WB IP ICC/IF
• Species Reactivities: Human, Monkey
• Antigen: Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro590 of human PIAS3 protein.
• Description: Rabbit Monoclonal
• Gene: PIAS3

Product images

Western blot analysis of extracts from RD cells, transfected with 100 nM SignalSilence ® Control siRNA (Unconjugated) #6568 (-), SignalSilence ® PIAS3 siRNA I #9073 (+), or SignalSilence ® PIAS3 siRNA II #9031 (+), using PIAS3 (D5F9) XP ® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). The PIAS3 (D5F9) XP ® Rabbit mAb confirms silencing of PIAS3 expression, while the β-Actin (D6A8) Rabbit mAb is used as a loading control.

Western blot analysis of extracts from various cell lines using PIAS3 (D5F9) XP ® Rabbit mAb.

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing full-length human PIAS3 (hPIAS3; +), using PIAS3 (D5F9) XP ® Rabbit mAb.

Confocal immunofluorescent analysis of A-204 (positive, left) and Hep G2 (low expression, right) cells using PIAS3 (D5F9) XP ® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).