Rat brain neural cultures stained with RBFOX3 / NEUN antibody (red), chicken polyclonal antibody to GFAP (green) and DNA (blue). The RBFOX3 / NEUN antibody antibody reveals strong nuclear and distal cytoplasmic staining for Fox3/NeuN and the complete absence of staining of astrocytes, which are staining with the GFAP antibody, and other kinds of non-neuronal cells. This Fox3/NeuN antibody is therefore an excellent marker of neuronal cells. Note that we have used the same immunogen to generate a rabbit polyclonal antibody to Fox3/NeuN, RPCA-Fox3.