PKCθ (E1I7Y) Rabbit mAb

• Supplier: Cell Signaling Technology
• Catalog: 13643
• Prices: $250.00
• Sizes: 100 µl (10 western blots)
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: E1I7Y
• Applications: WB IP IHC-P ICC/IF FC
• Species Reactivities: Human, Mouse, Rat, Bovine
• Antigen: Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro632 of human PKCθ protein.
• Description: Rabbit Monoclonal
• Gene: PRKCQ

Product images

Western blot analysis of extracts from wild-type and PKCθ (-/-) mouse splenocytes using PKCθ (E1I7Y) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). Extracts from wild-type and PKCθ (-/-) mouse splenocytes were kindly provided by Dr. Morgan Huse (Memorial Sloan-Kettering Cancer Center).

Western blot analysis of extracts from various cell lines using PKCθ (E1I7Y) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Immunoprecipitation of PKCθ from Jurkat cell extracts using Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (lane 2) or PKCθ (E1I7Y) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using PKCθ (E1I7Y) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human colon using PKCθ (E1I7Y) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human gastrointestinal stromal tumor (GIST) using PKCθ (E1I7Y) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human lymph node using PKCθ (E1I7Y) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded Jurkat (positive, left) or RL7 (negative, right) cell pellets using PKCθ (E1I7Y) Rabbit mAb.

Confocal immunofluorescent analysis of Jurkat (positive, left) and Raji (negative, right) cells using PKCθ (E1I7Y) Rabbit mAb (green). Blue pseudocolor= DRAQ5 ® #4084 (fluorescent DNA dye).

Flow cytometric analysis of mouse splenocytes using Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (left) and PKCθ (E1I7Y) Rabbit mAb (right). Splenocytes were co-stained with anti-CD3 APC and the Anti-rabbit IgG (H+L), F(ab') 2 Fragment (PE Conjugate) #8885 was used as a secondary antibody.