Detection of Human RIF1 by Immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-RIF1 ( Lot4 - left and Lot 3 - right) used at a dilution of 1:1000 (1 ug/ml). Detection: DAB.
Detection of Human RIF1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-RIF1 antibody used for IP at 6 ug/mg lysate. RIF1 was also immunoprecipitated by a previous lot of this antibody. For blotting immunoprecipitated RIF1, -4 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Detection of Human RIF1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-RIF1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.