RIF1

Gene Symbol RIF1
Entrez Gene 55183
Alt Symbol -
Species Human
Gene Type protein-coding
Description replication timing regulatory factor 1
Other Description RAP1 interacting factor homolog|rap1-interacting factor 1 homolog|telomere-associated protein RIF1
Swissprots Q9Y4R4 Q9H9B9 A6NC27 Q5UIP0 Q6PRU2 C9JBR1 Q99772 Q9NVP5 Q5H9R3 Q8TE94 Q9H830 Q66YK6 Q5UIP2
Accessions EAX11507 EAX11508 EAX11509 EAX11510 Q5UIP0 AK000323 AK001461 BAA91705 AK022932 BAB14313 AK024033 BAB14792 AK074349 BAB85058 AK299291 BAG61306 AK299572 BAG61511 AL080129 CAB45727 AL137363 AY584066 AAS94233 AY585745 AAT40745 AY727910 AAV51401 AY727911 AAV51402 AY727912 AAV51403 AY727913 AAV51404 BC072675 BC172393 AAI72393 BX648324 CR933663 CAI45961 U79263 AAB50209 XM_005246665 XP_005246722 XM_011511393 XP_011509695 XM_011511394 XP_011509696 XM_011511395 XP_011509697 XR_922954 XR_922955 XR_922956 XR_922957 NM_001177663 NP_001171134 NM_001177664 NP_001171135 NM_001177665 NP_001171136 NM_018151 NP_060621
Function Required for checkpoint mediated arrest of cell cycle progression in response to DNA damage during S-phase (the intra-S- phase checkpoint). This checkpoint requires activation of at least 2 parallel pathways by the ATM kinase: one involves the MRN (MRE11A-RAD50-NBS1) complex, while the second requires CHEK2. RIF1 seems to act independently of both these pathways. Seems to play no role in either the G1/S or G2/M DNA damage checkpoints. {ECO:0000269|PubMed:15342490}.
Subcellular Location Nucleus. Chromosome, telomere. Cytoplasm, cytoskeleton, spindle. Note=Exhibits ATM- and TP53BP1-dependent localization to uncapped or aberrant telomeres and to DNA double strand breaks (DSBs). Does not associate with normal telomere structures. Localizes to microtubules of the midzone of the mitotic spindle during anaphase, and to condensed chromosomes in telophase.
Tissue Specificity Highly expressed in testis. {ECO:0000269|PubMed:15583028}.
Top Pathways Signaling pathways regulating pluripotency of stem cells

Rif1 (N-20) - sc-55979 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Rif1 (N-20): sc-55979. Western blot analysis of RIF1 expression in non-transfected: sc-117752 (A) and human RIF1 transfected: sc-112277 (B) 293T whole cell lysates.

Rif1 (S-20) - sc-65195 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-Rif1 antibody - ab70254 from Abcam

Prices $378.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human laryngeal squamous cell carcinoma tissue labelling Rif1 with ab70254 at 1/1000 (1µg/ml). Detection: DAB.

Anti-Rif1 antibody - ab134812 from Abcam

Prices $376.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
Immunohistochemistry analysis of Formalin fixed and Paraffin Embedded (IHC-P) Human Testis tissue labelling Rif1 with ab134812 at 3.75 µg/ml.

Anti-Rif1 antibody - ab84785 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
ab84785, at 1/250 dilution, staining Rif1 in formalin-fixed, paraffin-embedded human seminoma by Immunohistochemistry using DAB staining.

Anti-RIF1 Antibody (aa1-50) - LS-C93305 from LifeSpan Bioscience

Prices $545.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-RIF1 Antibody (aa1425-1450) - LS-C93306 from LifeSpan Bioscience

Prices $545.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-RIF1 Antibody (C-Terminus) - LS-C93307 from LifeSpan Bioscience

Prices $545.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-RIF1 Antibody (C-Terminus) - LS-C93308 from LifeSpan Bioscience

Prices $665.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-RIF1 Antibody (aa2432-2444) - LS-C155014 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
RIF1 antibody (1 ug/ml) staining of HeLa and Mouse D3 (ES) lysates (35 ug protein in RIPA buffer). Data obtained from Hisao Masai, Tokyo Metropolitan Institute of Medical Science, Japan. Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-RIF1 Antibody (C-Terminus) - LS-C286452 from LifeSpan Bioscience

Prices $415.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human RIF1 by Immunohistochemistry. Sample: FFPE section of human seminoma. Antibody: Affinity purified rabbit anti-RIF1 used at a dilution of 1:250.

Anti-RIF1 Antibody (aa1425-1450) - LS-C287449 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human RIF1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from 293T (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HeLa (H; 50 ug) cells. Antibodies: Affinity purified rabbit anti-RIF1 antibody used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. RIF1 was also immunoprecipitated by the previous lot (-1) of this antibody. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

Anti-RIF1 Antibody (aa1-50) - LS-C287446 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human RIF1 by Immunohistochemistry. Sample: FFPE section of human laryngeal squamous cell carcinoma. Antibody: Affinity purified rabbit anti- RIF1 used at a dilution of 1:1000 (1 Detection: DAB.

Anti-RIF1 Antibody (C-Terminus) - LS-C287450 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human RIF1 by Immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-RIF1 ( Lot4 - left and Lot 3 - right) used at a dilution of 1:1000 (1 ug/ml). Detection: DAB.

Anti-RIF1 Antibody (aa2076-2089) IHC-plus™ - LS-B7340 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
Anti-RIF1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7340 concentration 3.75 ug/ml.

Anti-RIF1 Antibody (aa2406-2419) IHC-plus™ - LS-B1455 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-RIF1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1455 concentration 5 ug/ml.

Rif1 Antibody - NB100-1585 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry: Rif1 Antibody [NB100-1585] - Sample: FFPE section of human laryngeal squamous cell carcinoma. Antibody: Affinity purified rabbit anti- RIF1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Rif1 Antibody - NB100-1586 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry: Rif1 Antibody [NB100-1586] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- RIF1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).

Rif1 Antibody - NB100-58840 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry-Paraffin: Rif1 Antibody [NB100-58840] - Detection of Human RIF1 by Immunohistochemistry. Sample: FFPE section of human seminoma. Antibody: Affinity purified rabbit anti-RIF1 (NB100-58840 ) used at a dilutionof 1:250. Detection: DAB staining.

Rif1 Antibody - NB300-973 from Novus Biologicals

Prices $229.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

Anti-RIF1 (C-terminal) antibody produced in goat - SAB2502070 from Sigma-Aldrich

Prices $376.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-RIF1 (C-terminal) <B>Cat. No. SAB2502070</B>: Immunoblotting (1 μg/mL) staining of HeLa and Mouse D3 (ES) lysates (35 μg protein in RIPA buffer). Data obtained from Hisao Masai, Tokyo Metropolitan Institure of Medical Science, Japan. Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-RIF1 antibody produced in rabbit - HPA036887 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-RIF1: <B>Cat. No. HPA036887</B>: Immunohistochemical staining of human testis shows strong nuclear and cytoplasmic positivity in cells in seminiferus ducts.

RIF1-aa-2076-2089-Antibody-internal-region - AF2421a from Abgent, a WuXi AppTec company

Prices $325.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

RIF1-Antibody-C-Term - AF3774a from Abgent, a WuXi AppTec company

Prices $335.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
AF3774a (1 µg/ml) staining of HeLa and Mouse D3 (ES) lysates (35 µg protein in RIPA buffer). Data obtained from Hisao Masai, Tokyo Metropolitan Institure of Medical Science, Japan. Primary incubation was 1 hour. Detected by chemiluminescence.

RIF1 Antibody (OAEB00147) - OAEB00147 from Aviva Systems Biology

Prices $229.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Search more