Anti-RIF1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1455 concentration 5 ug/ml.
Anti-RIF1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1455 concentration 5 ug/ml.
![Anti-Rif1 Antibody - Western Blot. Western blot of Affinity Purified anti-Rif1 antibody shows detection of a band ~265 kD corresponding to mouse Rif1 (arrowhead). Specific reactivity with this band is blocked when the antibody is pre-incubated with the immunizing peptide (data not shown). Approximately 25 ug of MEF whole cell lysate was separated by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1.0 ug/ml for 2 h at room temperature followed by washes and reaction with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX ( for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.](http://www.bioprodhub.com/system/product_images/ab_products/3/sub_5/7870_62900_51417.jpg)
Anti-Rif1 Antibody - Western Blot. Western blot of Affinity Purified anti-Rif1 antibody shows detection of a band ~265 kD corresponding to mouse Rif1 (arrowhead). Specific reactivity with this band is blocked when the antibody is pre-incubated with the immunizing peptide (data not shown). Approximately 25 ug of MEF whole cell lysate was separated by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1.0 ug/ml for 2 h at room temperature followed by washes and reaction with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX ( for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.