Detection of Human RNF5 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from 293T (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HeLa (H; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-RNF5 antibody used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. RNF5 was also immunoprecipitated by rabbit anti-RNF5 antibody BL12866, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).