IHC of paraffin-embedded Carcinoma of Human liver tissue using anti-RSPO3 mouse monoclonal antibody. (heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
IHC of paraffin-embedded Human liver tissue using anti-RSPO3 mouse monoclonal antibody. (heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
IHC of paraffin-embedded Human Kidney tissue using anti-RSPO3 mouse monoclonal antibody. (heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
IHC of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-RSPO3 mouse monoclonal antibody. (heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RSPO3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RSPO3.