Western blot analysis of extracts from serum starved HeLa cells, untreated (-), insulin-treated (1 μM, 30 min; +), or insulin and λ phosphatase-treated (+), using Phospho-PRAS40 (Thr246) (D4D2) XP ® Rabbit mAb (upper) or PRAS40 Antibody #2610 (lower).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Phospho-PRAS40 (Thr246) (D4D2) XP ® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).
Immunohistochemical analysis of paraffin-embedded LNCaP cells, untreated (left) or LY294002-treated (right) using Phospho-PRAS40 (Thr246) (D4D2) XP ® Rabbit mAb. Cells used are from the SignalSlide ® Phospho-Akt (Ser473) IHC Controls #8101.
Immunohistochemical analysis of paraffin-embedded metastatic SK-OV-3 tumor in mouse lung using Phospho-PRAS40 (Thr246) (D4D2) XP ® Rabbit mAb.