Western blot analysis of extracts from NIH/3T3 cells, serum-starved for 14 hours and then either left untreated or treated with insulin (150 nM) for 15 minutes, using Phospho-PRAS40 (Thr246) Antibody.
Western blot analysis of extracts from NIH/3T3 cells, serum-starved for 14 hours and then either left untreated or treated with insulin (150 nM) for 15 minutes, using Phospho-PRAS40 (Thr246) Antibody. The experiment was performed in the presence of phospho-peptide specific to phospho-PRAS40 (Thr246) (upper panel), the corresponding nonphospho-peptide (middle panel) and in the absence of any blocking peptide (lower panel).