Western blot analysis of extracts from various cell lines using PREX1 (D8O8D) Rabbit mAb (upper) or GAPDH (D16H11) XP ® Rabbit mAb #5174 (lower). Differential expression of PREX1 in breast cancer cells is consistent with that described by Sosa, M.S. et al. (2010).
Confocal immunofluorescent analysis of MCF7 cells, treated with heregulin (10 ng/ml, 5 min; left) or heregulin (10 ng/ml, 5 min) and Wortmannin #9951 (1 μM, 1 hr; center), and MDA-MB-231 cells treated with heregulin (10 ng/ml, 5 min; right), using PREX1 (D8O8D) Rabbit mAb (green). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye).