Western blot analysis of extracts from various cell lines using PRMT4/CARM1 (3H2) Mouse mAb.
Immunoprecipitation of PRMT4/CARM1 from HCT 116 cell extracts using Mouse (G3A1) mAb IgG1 Isotype control #5415 (lane 2) or PRMT4/CARM1 (3H2) Mouse mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using PRMT4/CARM1 (C31G9) Rabbit mAb #3379.
Confocal immunofluorescent analysis of HeLa cells using PRMT4/CARM1 (3H2) Mouse mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 MCF7 cells grown in phenol red free medium and 5% charcoal stripped FBS for 4 d then treated with β-estradiol (10 nM) for 45 minutes and either 10 μl of PRMT4/CARM1 (3H2) or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP ® Human CCND1 Promoter Primers #12531, SimpleChIP ® Human ESR1 Promoter Primers #9673, SimpleChIP ® Human pS2 Promoter Primers #9702, and SimpleChIP ® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.