CARM1

Gene Symbol CARM1
Entrez Gene 10498
Alt Symbol PRMT4
Species Human
Gene Type protein-coding
Description coactivator-associated arginine methyltransferase 1
Other Description histone-arginine methyltransferase CARM1|protein arginine N-methyltransferase 4
Swissprots Q86X55 A6NN38
Accessions EAW84155 EAW84156 Q86X55 AF055027 AL833242 BC036886 BC046240 AAH46240 BC070496 BC156435 AAI56436 BC172490 AAI72490 BU176535 XM_005259708 XP_005259765 XM_011527637 XP_011525939 XM_011527638 XP_011525940 NM_199141 NP_954592
Function Methylates (mono- and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in several proteins involved in DNA packaging, transcription regulation, pre-mRNA splicing, and mRNA stability. Recruited to promoters upon gene activation together with histone acetyltransferases from EP300/P300 and p160 families, methylates histone H3 at 'Arg-17' (H3R17me), forming mainly asymmetric dimethylarginine (H3R17me2a), leading to activate transcription via chromatin remodeling. During nuclear hormone receptor activation and TCF7L2/TCF4 activation, acts synergically with EP300/P300 and either one of the p160 histone acetyltransferases NCOA1/SRC1, NCOA2/GRIP1 and NCOA3/ACTR or CTNNB1/beta-catenin to activate transcription. During myogenic transcriptional activation, acts together with NCOA3/ACTR as a coactivator for MEF2C. During monocyte inflammatory stimulation, acts together with EP300/P300 as a coactivator for NF-kappa-B. Acts as coactivator for PPARG, promotes adipocyte differenti
Subcellular Location Nucleus. Cytoplasm. Note=Mainly nuclear during the G1, S and G2 phases of the cell cycle. Cytoplasmic during mitosis, after breakup of the nuclear membrane.
Tissue Specificity Overexpressed in prostate adenocarcinomas and high-grade prostatic intraepithelial neoplasia. {ECO:0000269|PubMed:15221992}.

PRMT4/CARM1 (C31G9) Rabbit mAb - 3379 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone C31G9
Western blot analysis of extracts from HeLa and NIH/3T3 cells using PRMT4/CARM1 (C31G9) Rabbit mAb.

PRMT4/CARM1 (3H2) Mouse mAb - 12495 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Mouse
Clonality Monoclonal
Clone 3H2
Western blot analysis of extracts from various cell lines using PRMT4/CARM1 (3H2) Mouse mAb.

PRMT4/CARM1 Antibody - 4438 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of whole cell lysates from HeLa, NIH/3T3 and H-4-II-E cells using PRMT4/CARM1 Antibody.

CARM1 (G-2) - sc-393381 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
CARM1 (G-2): sc-393381. Western blot analysis of CARM1 expression in non-transfected: sc-117752 (A) and mouse CARM1 transfected: sc-119000 (B) 293T whole cell lysates.

CARM1 (H-140) - sc-33176 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
CARM1 (H-140): sc-33176. Western blot analysis of CARM1 expression in non-transfected: sc-117752 (A) and mouse CARM1 transfected: sc-119000 (B) 293T whole cell lysates.

CARM1 (D-6) - sc-390656 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
CARM1 (D-6): sc-390656. Western blot analysis of CARM1 expression in non-transfected: sc-117752 (A) and mouse CARM1 transfected: sc-119000 (B) 293T whole cell lysates.

CARM1 (M-16) - sc-5421 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
CARM1 (M-16): sc-5421. Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing nuclear staining of subset of cells in glomeruli and cytoplasmic staining of cells in tubules.

p-CARM1 (Ser 228) - sc-101649 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
Western blot analysis of phosphorylated CARM1 expression in EGF-treated A-431 whole cell lysate. Antibodies tested include p-CARM1 (Ser 228): sc-101649 preincubated with cognate phosphorylated peptide (A) and p-CARM1 (Ser 228): sc-101649 (B).

Anti-PRMT4 (phospho S228) antibody - ab194867 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PRMT4 (phospho S228) antibody (ab194867) at 1/500 dilutionLane 1 : A431 cell extractLane 2 : EGF treated A431 cell extract

Anti-PRMT4 antibody - ab128851 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PRMT4 antibody (ab128851) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate Lane 3 : MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell Lysate Lane 4 : HeLa Histone Preparation Nuclear Lysate Lane 5 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Lane 6 : PC3 (Human prostate carcinoma cell line) Whole Cell Lysate Whole Cell LysateLane 7 : DU 145 (Human prostate carcinoma cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-PRMT4 antibody - ab105449 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PRMT4 antibody (ab105449) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate Lane 3 : MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell LysateLane 4 : HeLa Histone Preparation Nuclear LysateLane 5 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell LysateLane 6 : PC3 (Human prostate carcinoma cell line) Whole Cell Lysate Whole Cell Lysate Lane 7 : DU 145 (Human prostate carcinoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-PRMT4 antibody - ab50214 from Abcam

Prices $378.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PRMT4 antibody (ab50214) at 1 µg/mlLane 1 : Nuclear extracts of MCF7 breast carcinoma cells Lane 2 : Nuclear extracts of MCF7 breast carcinoma cells with PRMT4 immunizing peptide at 10 µg/mlSecondaryGoat anti-Rabbit IgG, Peroxidase conjugatedeveloped using the ECL technique

Anti-PRMT4 antibody - ab131520 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PRMT4 antibody (ab131520) at 1/500 dilutionLane 1 : A431 cell extract untreatedLane 2 : A431 cell extract treated with EGF (200ng/ml, 5 mins)

Anti-PRMT4 antibody - ab84370 from Abcam

Prices $387.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
ab84370 at 1/100, staining human PRMT4 in a section of human breast adenocarcinoma by immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

Anti-PRMT4 antibody - ab87910 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-PRMT4 antibody [3H2] - ab110024 from Abcam

Prices $388.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 3H2
All lanes : Anti-PRMT4 antibody [3H2] (ab110024) at 1/500 dilutionLane 1 : MCF7 cell lysateLane 2 : Hela cell lysateLane 3 : NIH3T3 cell lysateLane 4 : HL-60 cell lysateLane 5 : LNcap cell lysateLane 6 : Jurkat cell lysateLane 7 : PC-3 cell lysateLane 8 : Cos7 cell lysateLane 9 : PC-12 cell lysate

Anti-PRMT4 / CARM1 Antibody (aa438-455) - LS-C3314 from LifeSpan Bioscience

Prices $265.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-PRMT4 / CARM1 Antibody (C-Terminus) - LS-C38318 from LifeSpan Bioscience

Prices $405.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-PRMT4 / CARM1 Antibody (Ser228) - LS-C51660 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-PRMT4 / CARM1 Antibody (aa225-275) - LS-C77770 from LifeSpan Bioscience

Prices $545.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-PRMT4 / CARM1 Antibody (aa450-500) - LS-C77771 from LifeSpan Bioscience

Prices $545.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-PRMT4 / CARM1 Antibody (C-Terminus) - LS-C77772 from LifeSpan Bioscience

Prices $545.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-PRMT4 / CARM1 Antibody (aa45-69) - LS-C641 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of PRMT4 in cell lysates from HeLa cells using antibody at 0.5 ug /ml. Twenty microgram of HeLa cell lysate was loaded per well of mini gel.

Anti-PRMT4 / CARM1 Antibody - LS-C125253 from LifeSpan Bioscience

Prices $425.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-PRMT4 / CARM1 Antibody - LS-C131563 from LifeSpan Bioscience

Prices $355.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
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