Detection of Human Selenoprotein S by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN buffer from HeLa cells. Antibodies: Affinity purified rabbit anti-Selenoprotein S antibody used for IP at 6 ug per reaction. Selenoprotein S was also immunoprecipitated by rabbit anti-Selenoprotein S antibody BL16285. For blotting immunoprecipitated Selenoprotein S, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of Human Selenoprotein S by Western Blot. Samples: Whole cell lysate (50 ug) prepared using NETN or RIPA buffer from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-Selenoprotein S antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.