Detection of Human SHMT1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-SHMT1 antibody used for IP at 6 ug per reaction. SHMT1 was also immunoprecipitated by rabbit anti-SHMT1 antibody BL15560. For blotting immunoprecipitated SHMT1, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of Mouse SHMT1 by Western Blot. Samples: Whole cell lysate (50 ug) from NIH3T3 cells. Antibodies: Affinity purified rabbit anti-SHMT1 antibody used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of Human SHMT1 by Western Blot. Samples: Whole cell lysate (50 ug) from Jurkat cells. Antibodies: Affinity purified rabbit anti-SHMT1 antibody used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.